Simplified primer design for PCR-based gene targeting and microarray primer database: two web tools for fission yeast
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چکیده
منابع مشابه
Simplified primer design for PCR-based gene targeting and microarray primer database: two web tools for fission yeast
PCR-based gene targeting is a popular method for manipulating yeast genes in their normal chromosomal locations. The manual design of primers, however, can be cumbersome and error-prone. We have developed a straightforward web-based tool that applies user-specified inputs to automate and simplify the task of primer selection for deletion, tagging and/or regulated expression of genes in Schizosa...
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Tetra-primer ARMS-PCR is used extensively as a low cost, single PCR assay requiring no post-PCR manipulation. The design of successful primers depends on a number of variables such as melting temperatures, GC content, complementarity and selection of mismatch bases. The optimal selection of primers can be achieved in an automated way using a program which evaluates candidate primers for a given...
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SUMMARY The visualization of gene structure plays an important role in polymerase chain reaction (PCR) primer design, especially for eukaryotic genes with a number of splice variants that users need to distinguish between via PCR. Here, we describe a visualized web server for primer design named VizPrimer. It utilizes the new information technology (IT) tools, HTML5 to display gene structure an...
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ژورنال
عنوان ژورنال: Yeast
سال: 2006
ISSN: 0749-503X,1097-0061
DOI: 10.1002/yea.1422